DGG Mannequin

Department of General, Visceral, Thoracic and Vascular Surgery

Research location

Department of General, Visceral, Thoracic and Vascular Surgery
Head of the Clinic: Prof. Dr S. Kersting

Research

Greifswald University Hospital – a public-law corporation –

Clinic and Outpatient Clinic for General Surgery, Visceral, Thoracic and Vascular Surgery

Department: Surgical Research Laboratory DZ7

Location
Ferdinand-Sauerbruch-Straße
17475 Greifswald

Icon Ausstattung

Equipment

Basic Research Laboratory: Molecular Biology and Other Fields

Small animal laboratory

Icon Methoden

Methods

  • Cell culture
  • FACS
  • Various in-house enzyme-linked immunosorbent assays, radioimmunoassays, luminescence immunoassays
  • Detection of antibodies/cytokines against implanted vascular prostheses
  • Implantations/explantations in rats and mice (also in pigs and sheep in collaboration)
  • Histology
  • TUNEL staining, fluorescence microscopy
  • Immunohistochemistry, immunofluorescence
  • Morphometry
  • Digital image analysis systems KS400, ImageJ, QuPath
  • Multiplex assays for differentiated cytokine/chemokine analysis
Icon Personal

Staff

  • Doctors involved in research projects            
  • TAs
  • PhD students
  • PhD
  • Scientists

Research focus areas

Clinical: Projects on amputation criteria

Translational: Projects on Limb Ischaemia, Peripheral Ischaemia

Basic research: Projects: Biocompatibility of medical implants, currently HOGEMA

Contact person

Prof. Dr. med. Andreas Hoene

Send email

Collaborations

On campus: Leibniz Institute for Plasma Science and Technology

Interregional: University of Rostock (orthopaedics, cell biology, etc.), Wismar University of Applied Sciences

Events and network

on site

Publications

  • 7 Ongoing PhD theses
  • 4 completed doctoral theses from the last 5 years (2017–2021)
  • 4 completed postdoctoral theses from the last 10 years (2012–2021)

Publications

  1. Schlosser M, Wilhelm L, Urban G, Ziegler B, Ziegler M, Zippel R. Immunogenicity of polymeric implants: Long-term antibody response against polyester (Dacron) following the implantation of vascular prostheses into LEW.1A rats. J. Biomed Mater Res 61 (2002) 450–457. (IF: 2.462)
  2. Zippel R, Wilhelm L, Marusch F, Koch A, Urban G, Schlosser M. Antigenicity of polyester (Dacron) vascular prostheses in an animal model. Eur J Vasc Endovasc Surg 21 (2001) 202–207. (IF: 1.484)
  3. Schlosser M, Zippel R, Hoene A, Urban G, Ueberrueck T, Marusch F, Koch A, Meyer L, Wilhelm L. Antibody response to collagen after functional implantation of different polyester vascular prostheses in pigs. J Biomed Mater Res A 72 (2005) 317–325. (IF: 2.743)
  4. Zippel R, Hoene A, Walschus U, Jarchow R, Ueberrueck T, Patrzyk M, Schlosser M, Wilhelm L. Digital image analysis for morphometric evaluation of tissue response after implanting alloplastic vascular prostheses. Microscopy & Microanalysis 12 (2006) 366–375. (IF: 2.108)
  5. Wilhelm L, Zippel R, von Woedtke T, Kenk H, Hoene A, Patrzyk M, Schlosser M. Immune response against polyester implants is influenced by the coating substances. J Biomed Mater Res A 83 (2007) 104–113. (IF: 2.497)
  6. Zippel R, Wilhelm L, Hoene A, Walschus U, Ueberrueck T, Schlosser M. Local tissue reaction and differentiation of the prosthesis-specific antibody response following functional implantation of vascular grafts in pigs. J Biomed Mater Res B: Appl Biomaterials 85 (2008) 334–353. (IF: 2.030)
  7. Walschus U, Goldmann H, Ueberrueck T, Hoene A, Wilhelm L, Schlosser M. Evaluation of the biocompatibility of a new vascular prosthesis coating by detection of prosthesis-specific antibodies. Journal of Materials Science: Materials in Medicine 19 (2008) 1595–1600. (IF: 1.508)
  8. Patrzyk, M, Hoene A, Jarchow R, Wilhelm L, Walschus U, Zippel R, Schlosser M. Time course of fibronectin in the peri-implant tissue and neointima formation after functional implantation of polyester-based vascular prostheses with different porosity in pigs. Biomed Mater 5 (2010) 055003. (IF: 2.467)
  9. Hoene A, Walschus U, Patrzyk M, Finke B, Lucke S, Nebe B, Schroeder K, Ohl A, Schlosser M. In vivo investigation of the inflammatory response against allylamine plasma polymer-coated titanium implants in a rat model. Acta Biomater 6 (2010) 676–683. (IF: 4.824)
  10. Walschus U, Hoene A, Kochanowski A H, Neukirch B, Patrzyk M, Wilhelm L, Schroeder K, Schlosser M. Quantitative immunohistochemical examination of the local cellular reactions following implantation of biomaterials. J of Microscopy 242 (2011) 94–99. (IF: 1.631)
  11. Walschus U, Hoene A, Patrzyk M, Finke B, Polak M, Lucke S, Nebe B, Schröder K, Podbielski A, Wilhelm L, Schlosser M. Serum profile of pro- and anti-inflammatory cytokines in rats following implantation of low-temperature plasma-modified titanium plates. J Mater Sci Mater Med 23 (2012) 1299–1307. (IF: 2.141)
  12. Hoene A, Patrzyk M, Walschus U, Straňák V, Hippler R, Testrich H, Meichsner J, Finke B, Rebl H, Nebe B, Zietz C, Bader R, Podbielski A, Schlosser M. In vivo examination of the local inflammatory response following implantation of Ti6Al4V samples treated with a combined low-temperature plasma process involving pulsed magnetron sputtering of copper and plasma-polymerised ethylenediamine. J Mater Sci Mater Med 24 (2013) 761–771. (IF: 2.379)
  13. Hoene A, Prinz C, Walschus U, Lucke S, Patrzyk M, Wilhelm L, Neumann HG, Schlosser M. In vivo evaluation of copper release and acute local tissue reactions following implantation of copper-coated titanium implants in rats. Biomed Mater. 8 (2013) 035009. (IF: 2.922)
  14. Lucke S, Hoene A, Walschus U, Kob A, Pissarek J-W, Schlosser M. Acute and chronic local inflammatory reaction following implantation of different extracellular porcine dermal collagen matrices in rats. BioMed Research International 2015; 15:938059 doi: 10.1155/2015/938059. (IF: 2.134)
  15. Hoene A, Patrzyk M, Walschus U, Finke B, Lucke S, Nebe B, Schröder K, Schlosser M. Systemic IFNγ predicts local implant macrophage response. J Mater Sci Mater Med. 2015 Mar;26(3):131. doi: 10.1007/s10856-015-5476-5 (IF: 2.272)
  16. Walschus U, Hoene A, Patrzyk M, Lucke S, Finke B, Polak M, Lukowski G, Bader R, Zietz C, Podbielski A, Nebe JB, Schlosser M. A cell-adhesive plasma polymerised allylamine coating reduces the in vivo inflammatory response induced by Ti6Al4V modified with plasma immersion ion implantation of copper. J Funct Biomater 8 (2017) 30; doi:10.3390/jfb8030030 (IF: 4.35)
  17. Lucke S, Walschus U, Hoene A, Schnabelrauch M, Nebe JB, Finke B, Schlosser M. The in vivo inflammatory and foreign body giant cell response against different poly(l-lactide-co-d/l-lactide) implants is primarily determined by material morphology rather than surface chemistry. J Biomed Mater Res A 106 (2018) 2726–34 (IF: 3.221)
  18. Hoene A, Lucke S, Walschus U, Hackbarth C, Prinz C, Evert FK, Neumann HG, Schlosser M. Effects of copper-impregnated collagen implants on local pro- and anti-inflammatory and regenerative tissue reactions following implantation in rats. J Biomed Mater Res A 108 (2020) 871–881. (IF: 4.396)
  19. Koppe C, Hoene A, Walschus U, Finke B, Testrich H, Pohl C, Brandt N, Patrzyk M, Meichsner J, Nebe B, Schlosser M. Local Inflammatory Response after Intramuscular Implantation of Anti-Adhesive Plasma-Fluorocarbon-Polymer Coated Ti6Al4V Discs in Rats. Polymers (Basel). 13 (2021) 2684. doi: 10.3390/polym13162684. (IF: 4.329 for 2020)
  20. Köhler R, Pohl C, Walschus U, Zippel R, Wilhelm L, Hoene A, Patrzyk M, Schlosser M. Association of systemic antibody response against polyethylene terephthalate with inflammatory serum cytokine profile following implantation of differently coated vascular prostheses in a rat animal model. J Biomed Mater Res A. 9 July 2021. doi: 10.1002/jbm.a.37265. Online ahead of print. (IF: 4.396 for 2020)
Back to list